Spinning disk confocal, Nikon W1
Skirball 4th floor

Signup to use microscope
BEFORE 15 AUG 2024:  http://signups.med.nyu.edu/facilities/equipment/signups/skirball-spinning-disk
AFTER 14 AUG 2024: https://nyulangonesignups.resourcescheduler.net/resourcescheduler/scheduleWeek.asp?Topic=RES&TopicID=84&View=week&

Instructions:  [Word doc]   [PDF]       How to set up a multipoint Z series time series.

This microscope is for fluorescence imaging. Primary use is spinning disk confocal. Can be used in bypass mode for widefield.

A few of the specifications:

• Apo LWD 20X 0.95 Water λS DIC N2 adjustable collar 0.11 to 0.23
• Apo LWD 40X 1.15 Water λS DIC N2 adjustable collar 0.15 to 0.19
  
• Plan Fluor 40X 1.3 Oil 0.17
• Apo 60x 1.40 Oil λS DIC N2 0.13 WD
• SR HP Plan Apo 100X 1.35 Silicon Oil λS DIC N2 adjustable collar 0.15-0.19 and temperature
  All lenses with collars, match the thickness of coverslip or adjust empirically to maximize resolution.
  
  Chromatic aberration test results here.
   
  
• Emission filter wheel positions:
  1. 455/50
  2. 525/36
  3. 540/24
  4. 605/52
  5. 705/72
  6. 89101 quad 405/488/555/640
  7?. 525/50
  two empty positions
   
  
• Laser wavelengths for confocal: 405, 488, 561, 640 nm
• LED wavelengths for widefield: 360, 488, 561, 640, 730 nm
   
• Nomarski/DIC optics for 60X

• Camera beamsplitter 565nm - aligning beamsplitter instructions
• 2 matched Andor 888 Live EMCCD cameras - notes on advanced settings - methods to lock display contrast
• Galvo based scanner for FRAP/photconversion/bleaching/ablation.
• Piezo Z stage with 220 um travel * settings for Z series here
  
  
• Tokai Hit incubation system STXG-TIZWX-SET SN 190525 shipping date 20190307 Instructions here.

Troubleshooting hardware? Try reviewing the original installation values posted here.
As of Dec 2019, installation files and othe backups are stored here:
R:\MicroscopyCore\mcammer\equipment profiles\Nikon spinning disk\default_settings_after_201912_reinstall
which all Microscopy Core staff have permissions to read.

Example image at left and brief tour of the microscope at right.

Click on image for full field version in new window.