Spinning disk confocal, Nikon W1
Skirball 4th floor


Signup to use microscope.  http://signups.med.nyu.edu/facilities/equipment/signups/skirball-spinning-disk


Instructions:  [Word doc]   [PDF]


This microscope is for fluorescence imaging. Primary use is spinning disk confocal. Can be used in bypass mode for widefield.

A few of the specifications:

  • Apo LWD 20X 0.95 Water λS DIC N2 adjustable collar 0.11 to 0.23
  • Apo LWD 40X 1.15 Water λS DIC N2 adjustable collar 0.15 to 0.19
  • Plan Fluor 40X 1.3 Oil 0.17
  • Apo 60x 1.40 Oil λS DIC N2 0.13 WD
  • SR HP Plan Apo 100X 1.35 Silicon Oil λS DIC N2 adjustable collar 0.15-0.19 and temperature
    All lenses with collars, match the thickness of coverslip or adjust empirically to maximize resolution.
     
  • Emission filter wheel positions:
    1. 455/50
    2. 525/36
    3. 540/24
    4. 605/52
    5. 705/72
    6. 89101 quad 405/488/555/640
    7. blank
     
  • Laser wavelengths for confocal: 405, 488, 561, 640 nm
  • LED wavelengths for widefield: 360, 488, 561, 640, 730 nm
  • Camera beamsplitter 565 LP (or SP? check this - insert drawing of light paths)
  • 2 matched Andor 888 Live EMCCD cameras - notes on advanced settings

 

Click on image for full field version in new window.

 

 

 

 


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