A few examples of FRAP that we have done.
FRAP of lipid bilayers by widefield illumination. This method requires the user to very rapidly move parts on the microscope. A motorized scope with a software script could be more uniform. 2011.
Example of FRAP in neuron cell culture using Nikon A1 in 2017
Similar microscope setup and analysis as FRAP, localized photodamage or photoactivation. Nikon A1, 2017.
Pre-2001 BioRad confocal instructions for FRAP.
Zeiss 880 confocal FRAP
See:
Zeiss 880 and 710 and 700 confocals and Leica SP8 confocal
Spinning disk confocal with miniscanner