Leica Stellaris with white light & MaiTai lasers Leica Stellaris 8 Falcon laser scanning confocal microscope on inverted DMi8 CS stand with Super Z Galvo and resonant scanner, giving complete spectral freedom to choose probe wavelengths from 440 to 790 nm with a tunable third-generation white light laser, a 405 nm laser, four sensitive HyD detectors, fluorescence lifetime (FLIM) capability and imaging across the spectrum from 450 to 850 nm. Optics will accommodate an existing MaiTai DeepSee laser for second harmonics imaging. Objective lenses from 10X to 63X, an Okolab environmental chamber for use with live cells and tissues, and workstation and Stellaris modules for complex instrument operation, post-processing, and analysis included. The Stellaris was purchased to fulfill a wide range of confocal based imaging needs. Of course, this is an exceptional instrument for standard confocal, but this microscope has unique features, such as time domain fluorescence lifetime (FLIM) to complement the lower resolution frequency domain Toggel FLIM system. Why FLIM?
Consider using the resonant scanner for faster image collection, especially when tiling large areas with Z series. For excitation of fluorescence, any wavelengths may be chosen from 440 nm up to 1040 nm. From 440 to 790 nm, up to eight laser lines maybe chosen simultaneously and a MaiTai DeepSee may be used for a single wavelength in the range 760 to 1040 nm for second harmonics or 2 Photon using internal detectors with the pinhole open. This microscope does image bright 2P signal, but for most intravital imaging, we continue to recommend the Olympus FVMPE-RS. There is also a standard 405 nm diode laser. Scanning is standard galvos or faster resonant. (This system does not allow for simultaneous use of galvos and resonant scanner, such as for highly localized photoactivation with galvos while scanning full field resonant.) |
Signup to use microscope. Projects requiring unique functions of this microscope have priority. Please use other confocals for standard confocal work. |
Instructions:
At this time, there is no iLab Kiosk required to startup. However, it will likely be implemented. [Word doc] [PDF]
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A few of the specifications:
Lenses -- We chose these lenses for specfic reasons; click here for explanations.
Four detectors for low light plus tramsmitted light detector.
Additional PARTS ORDER LIST: 10 158301100 1 STELLARIS 8 20 158301134 1 8k Tandem Scanner STELLARIS 8 DIVE/CRS 30 158204510 1 Fluorifier Disc Basis inkl. Analysator 40 158204512 1 Notch filter set VIS full 50 158301124 1 Scan optics VISIR with rotation for DMi8 60 158201310 1 SP Light Trap 110 158305400 1 Pulse trigger interface 120 158301140 1 STELLARIS 8 DMi8 Adapter 130 158301200 1 Laser Port 405/UV 140 158202140 1 Laser 405 DMOD 150 155933663 1 DMi8 CS Premium 180 158204202 1 Closed Loop Focus with AFC 200 158000677 1 Leica LED 3 210 158004141 1 Scanning stage inv. universal 230 15525226 1 XY advanced board for scanning stage 240 158004421 1 SuperZ Galvo Stage 250 158004424 1 Insert Universal Inverse SuperZ 260 15525309 1 Filter Cube DsRed 270 15525314 1 Filter Cube GFP 280 15525338 1 Filter cube LED 405 390 15555019 1 IC Condenser Prism K5 400 15555006 1 IC Prism A 410 15521524 1 IC Condenser Prism K10 420 15555018 1 IC Condenser Prism K4 430 15555056 1 IC Prism D1 440 15555017 1 IC Condenser Prism K3 450 15555046 1 IC Prism E 460 158206045 1 Okolab Laser Safety Box DMi8 4TUNE 470 158206043 1 Okolab Temp. Ctrl Laser Safety Box 480 158206037 1 Okolab Sample Chamber Inv Super Z 490 158206031 1 Okolab Insert multi well plate SuperZ 500 158206040 1 Okolab CO2, Hypoxia, Humidity Passive 505 158206042 1 Laser Safety Kit Okolab 510 158003151 2 High Brilliance Monitor flat 520 158203113 1 Workstation Expert 540 158203200 1 LAS X STELLARIS Control Software 550 158203204 1 LAS X Lightning Expert 560 158203206 1 LAS X Dye Finder 570 158203208 1 LAS X FRAP Zoomer 580 158203211 1 LAS X Assay Editor 590 158203214 1 LAS X Environmental Control 600 158003206 1 LAS X MicroLab 601 158203207 1 LAS X MicroLab 610 27100001 1 Aivia 3D 620 158305100 1 STELLARIS 8 FALCON 630 158305101 1 STELLARIS 8 FCS 690 158004512 1 MP Barrier Filter Set 700 158002641 1 Single beam routing with VBE 720 158002640 1 IR Laser attenuation AOM 730 158301212 1 IR multifunction port DIVE inverse 740 158005232 1 Tau excitation DIVE |
Recommended alternative to water for using water immersion objectives.
General Confocal Best practices:
Always save files as LIF.
Other Stuff
MaiTai laser control manually, for instance if LASX does not turn off the laser. Notes from first day of training. .rtf .pdf
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SN 8300000373 MaiTai HPDS-OL MAR2018 S/N 20479
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