Zeiss 880 Laser Scanning Confocal microscope with FCS, FLIM, adn AiryScan
Skirball 2nd floor


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Instructions starup/shutdown/general imaging:  [Word doc]   [PDF]  
(coming soon - for now look at link at bottom of this page)

Instructions AiryScan:  [Webpage]  

 

This microscope is most commonly used for standard fluorescence confocal. It also has other imaging modes, a few listed here:

  • reflection, IRM
  • transmitted light
  • FLIM (time domain, excitation with pulsed 440 nm laser)
  • FCS and FCCS (continuous wavelength excitation)

A few of the specifications:

  • Zeiss AxioObserver.inverted stand
  • environmental chamber for temperature and stage insert for CO2 control
  • 10X 0.3
  • 20X Plan-Apochromat 0.8 M27
  • 40X
  • 40X
  • 63X 1.40
  • 100X Use only for Airyscan and other specialty work. NOT FOR ROUTINE CONFOCAL Instead, use the 63X witha zoom set or large pixel size image.
  • Excitation lasers: 405, 440, 458, 488, 514, 561, 594, 633 nm
  • Detectors, path 1:
    * PMT, 410 to 750 nm
    * 32 channel spectral, 409 to 695 nm
    * redshifted GaAsP, up to 750 nm
    * transmitted detector
  • Detectors, path 2:
    * 2 GaAsP detectors ("BIG" detectors) with standard filter blocks for green/red and CFP/YFP
  • Detector, path 3:
    * AiryScan
  • Automated Stage (tiling, repeated multiple positions)
  • Auto focus


 

Other links

 

 


Old website here for reference: indexpre20180701.html


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