Zeiss 700 microscope
Alexandria Center 8th Floor, room 815

To use microscope you must be trained by Michael Cammer or Yan Deng via the Microscopy Core.

** Signup to use microscope ** Must use Safari, Chrome, or Firefox; not Explorer

Quickstart Guide

Switches #1, #2, and #3.
  1. Must have appointment in iLab.
  2. Only if need heater in environmental chamber, power strip #3 for incubator.
  3. Hg arc lamp for viewing fluorescence by eye.
  4. Power strip switch #1.
  5. Power strip switch #2.
  6. Button for computer.


  1. Copy your files over network or to USB device to take with you.
  2. Check whether someone is coming in soon after you.
  3. Quit software.
  4. Shut down computer.
  5. Switches in reverse order.
  6. Sign out logbook.

General Confocal Best practices:

About the microscope

Laser lines Useful for...
405 nm Dapi, CFP, Pacific Blue, Alexa 405, Brilliant Violets
488 nm FITC, Alexa488, GFP, YFP, NBD
555 nm RFP, mCherry, rhodamine, Cy3, Alexa568, Alexa594, propidium iodide
639 nm Cy5, Alexa647, To-Pro


There are two ways to choose the spectral detection. One is by filter and the other is by a user adjustable mask. If you are using multiple channels, imaging will be much faster if the mask does not move between channels.

Emission filters Useful for...
  Dapi, CFP, Pacific Blue
  FITC, Alexa488, GFP, YFP; (May collect spillover from blue)
  RFP, mCherry, rhodamine, Cy3, Alexa568, Alexa594, propidium iodide; (May collect spillover from green)
  RFP, mCherry, rhodamine, Cy3, Alexa568, Alexa594, propidium iodide
  Cy5, Alexa647, To-Pro; (May collect spillover from red)




Laser Power
Diode 405 nm ? mW

Diode 488 nm

? mW
Diode 555 nm ? mW
Diode 639 nm ? mW




Manual uncaging / photo activation using widefield illumination.


Publications with data from this microscope:



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comments, questions, suggestions for this web page: Michael.Cammer@med.nyu.edu or mcammer@gmail.com