Zeiss 700 microscope
Alexandria Center 8th Floor
To use microscope you must be trained by Michael Cammer or Yan Deng via the Microscopy Core.
Signup to use microscope
Instructions are a work in progress. For most recent version:
Switches #1, #2, and #3.
About the microscope
|Laser lines||Useful for...|
|405 nm||Dapi, CFP, Pacific Blue|
|488 nm||FITC, Alexa488, GFP, YFP|
|555 nm||RFP, mCherry, rhodamine, Cy3, Alexa568, Alexa594, propidium iodide|
|639 nm||Cy5, Alexa647, To-Pro|
There are two ways to choose the spectral detection. One is by filter and the other is by a user adjustable mask. If you are using multiple channels, imaging will be much faster if the mask does not move between channels.
|Emission filters||Useful for...|
|Dapi, CFP, Pacific Blue|
|FITC, Alexa488, GFP, YFP; (May collect spillover from blue)|
|RFP, mCherry, rhodamine, Cy3, Alexa568, Alexa594, propidium iodide; (May collect spillover from green)|
|RFP, mCherry, rhodamine, Cy3, Alexa568, Alexa594, propidium iodide|
|Cy5, Alexa647, To-Pro; (May collect spillover from red)|
|Diode 405 nm||? mW|
Diode 488 nm
|Diode 555 nm||? mW|
|Diode 639 nm||? mW|
Manual uncaging / photo activation using widefield illumination.
comments, questions, suggestions for this web page: Michael.Cammer@med.nyu.edu or firstname.lastname@example.org