Zeiss 700 microscope
Alexandria Center 8th Floor

To use microscope you must be trained by Michael Cammer or Yan Deng via the Microscopy Core.


Signup to use microscope

 

Instructions are a work in progress. For most recent version:

 

Quickstart Guide


Switches #1, #2, and #3.
  1. Sign in logbook
  2. Only if need heater in environmental chamber, power strip #3 for incubator.
  3. Hg arc lamp for viewing fluorescence by eye.
  4. Power strip switch #1.
  5. Power strip switch #2.
  6. Button for computer.

Shutdown:

  1. Copy your files over network or to USB device to take with you.
  2. Check whether someone is coming in soon after you.
  3. Quit software.
  4. Shut down computer.
  5. Switches in reverse order.
  6. Sign out logbook.

About the microscope

Laser lines Useful for...
405 nm Dapi, CFP, Pacific Blue
488 nm FITC, Alexa488, GFP, YFP
555 nm RFP, mCherry, rhodamine, Cy3, Alexa568, Alexa594, propidium iodide
639 nm Cy5, Alexa647, To-Pro

 

There are two ways to choose the spectral detection. One is by filter and the other is by a user adjustable mask. If you are using multiple channels, imaging will be much faster if the mask does not move between channels.

Emission filters Useful for...
  Dapi, CFP, Pacific Blue
  FITC, Alexa488, GFP, YFP; (May collect spillover from blue)
  RFP, mCherry, rhodamine, Cy3, Alexa568, Alexa594, propidium iodide; (May collect spillover from green)
  RFP, mCherry, rhodamine, Cy3, Alexa568, Alexa594, propidium iodide
  Cy5, Alexa647, To-Pro; (May collect spillover from red)

 

Lenses:

 

Laser Power
Diode 405 nm ? mW

Diode 488 nm

? mW
Diode 555 nm ? mW
Diode 639 nm ? mW

 

 

 

 

Manual uncaging / photo activation using widefield illumination.

 

 

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comments, questions, suggestions for this web page: Michael.Cammer@med.nyu.edu or mcammer@gmail.com