Multifield Timelapse with Z series

Z series with multiple positions only works with a center position set and all Z series must have the same number of slices, step size, etc.

In the upper left experiment window you need to check Z, timelapse, and positions.

(click here for full screen snap)

Make sure the time interval is longer than the time required to take Z series at all positions.


Saving files

At the end of the experiment, save the entire experiment in CZI format.

We recommend saving files during the image collection too.

Zeiss recommends using Streaming. The example above uses Auto Save. We prefer the latter. The reason is that Streaming creates files that are reliant on the first saved file and difficult to use with ImageJ. Auto Save saves each file autonomously and is easy to use with ImageJ including during the experiment.