Problem:
A need to use the Opal Multiplex Immunohistochemistry with the Zeiss 880 confocal and clearly separate signals.
We needed to see more spectral detail than the table published on the Perkinelmer website.
Opal Fluorophores |
Wavelength |
|
Excitation |
Emission |
|
Opal 520 |
494 nm |
525 nm |
Opal 540 |
523 nm |
536 nm |
Opal 570 |
550 nm |
570 nm |
Opal 620 |
588 nm |
616 nm |
Opal 650 |
627 nm |
650 nm |
Opal 690 |
676 nm |
694 nm |
Spectral DAPI |
358 nm |
461 nm |
Therefore, Experimental Pathology Research Laboratory at NYU Langone Medical Center prepared a sample of each probe and we measured with Zeiss 880 confocal in lambda mode, Plan-Apochromat 20x/0.8 M27, pinhole open.
Full spreadsheet in Excel format here.