Example 3D movie.
Originally generated in Imaris 9.6 and frames exported as folders of tif or png files.
These were reassembled in ImageJ with text added (including fading out) and exported to tif or uncompressed avi.
Mp4 conversion in free Windows movie maker or Adobe Premier. No lossy compression until the final export step.
Raw data 4 channel
spinning disk confocal using 60x N.A. 1.4 oil lens with pixel sizes 0.22 um in XY using an Andor 800 camera (lik to web page). Z step at 0.3 um. An alternate lens we liked for this project was 100X N.A. 1.3 silicon oil.
Objects obscuring the features of interest were filtered after segmentation in Imaris 9.6 but are visible in the 2D fluorescence images for full reference.