Zeiss 700 upright microscope SK5
To use this scope please contact Michael Cammer & Yan Deng and have permission from Dr. Steve Burden.
Signup to use microscope http://signups.med.nyu.edu/facilities/equipment/signups/zeiss-700-confocal-5th-floor
All files must be saved on Drive D:
All files should be copied to Medical Center maintained server.
If you are asked to install an update, just say no!
About the microscope
|Laser lines||Useful for...|
|405 nm||Dapi, CFP, Pacific Blue|
|488 nm||FITC, Alexa488, GFP, YFP|
|555 nm||RFP, tdTomato, mCherry, rhodamine, Cy3, Alexa568, Alexa594, propidium iodide, DiI|
|639 nm||Cy5, Alexa647, miRFP670, To-Pro|
There are two ways to choose the spectral detection. One is by filter and the other is by a user adjustable mask. If you are using multiple channels, imaging will be much faster if the mask does not move between channels.
Use oil that says RI 1.518 on the bottle.
For best results, use #1.5 coverslips as explained here.
Sign in Logbook!!
Example 3D from this microscope made with ImageJ.
comments, questions, suggestions for this web page: Michael.Cammer@med.nyu.edu or email@example.com