Zeiss 700 upright microscope SB 4th floor

To use this scope please contact Michael Cammer & Yan Deng and have approval of Dept Cell Bio faculty

Signup to use microscope: https://signups.med.nyu.edu/facilities/equipment/signups/zeiss-700-confocal-science-building-4th-floor

All files must be saved on Drive D:
All files should be copied to Medical Center maintained server.

If you are asked to install an update, just say no!

About the microscope

There are two ways to choose the spectral detection. One is by filter and the other is by a user adjustable mask. If you are using multiple channels, imaging will be much faster if the mask does not move between channels.

Lenses:

• 10X plan neofluar N.A. 0.30
• 20X planapochromat N.A. 0.8
  
• 40X planapochromat N.A. 1.4

Use oil that says RI 1.518 on the bottle.

For best results, use #1.5 coverslips as explained here.

Startup:

Sign in Logbook!!

General Confocal Best practices:

• The pinhole is what makes the confocal a confocal. Always set at 1AU (which means 1 Airy unit) and click the 1AU button each time you change lenses.
  If you are opening it for imaging fixed samples, you should go use a widefield fluorescence scope instead.
  Except in exceptional live cell imaging where you understand that you are not being confocal, this is NOT AN ACCEPTABLE WAY TO MAKE IMAGES BRIGHTER. You won't hurt the instrument, but when you write your methods, you won't be accurately describing your microscopy as "confocal".
• Offset. Always use at 0 or 1.
  Other numbers are wrong.
• Digital gain. The preset is 0 or 1. Leave it there.
• Example of scan speed, averaging, filtering and noise click here.

Shutdown:

1. Quit software.
2. Transfer your files to server space.
3. Shut down computer.
4. Switches in reverse order.
5. Sign out of Logbook

Example 3D from this microscope made with ImageJ.

LSM700 SN 2601001055
1-800-633-6610 1 1

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comments, questions, suggestions for this web page: Michael.Cammer@med.nyu.edu or mcammer@gmail.com