Crest spinning disk on Nikon Ti2 stand
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All samples for this microscope require a #1.5 coverslip. Read more here.
Seven laser lines.
More details coming soon.
All data must be stored on drives D:, E:, F:.
If you store data on drive C: or on the desktop, you may lose access to the scope.
If you plug a USB device in that has not been MCIT anti-virus scanned and vetted by core staff in advance, you also may lose access to the scope.
Drive D: must be empty at the end of your session. It is a solid state drive for highest speed imaging. You may collect your files here but need to copy them to E: or F: at the send of your session.
Files should be copied or moved to the server before the end of every session. Official policy is that data may not be left on E: or F:, but for practial purposes, this is tolerated until disk space drops below 1 TB. When disk space drops below 1 TB, files will be deleted oldest to most recent. There will be no warnings. Each user is responsible for their data.
The Kinetix camera is very low noise. You may find that setting the maximum intensity at 6000 in 16 bits mode is more than enough signal. The camera has a very large field of view and a few modes. Most people will use it in the 16 bits mode. However, understanding the different modes is important if you want to approach single molecule type sensitivity or drive the camera fast, such as 500 frames per second. More details here.
FOV (Field Of View):
This means how large an image can you take. The largest image is 2720 X 2720 pixels. With the 40X lens, this covers 442 X 442 um. At 100X, the FOV is 177 X 177 um. Of course, you may choose a smaller FOV. Read more here.