Nikon Eclipse Ti microscope Ideal for:
This microscope is for widefield fluorescence, brightfield with a grayscale camera, and phase contrast. It has automated focus for Z series, focus stabilzation for time lapse, and precision stage for tiling large areas or repeatably imaging multiple positions. It may be used for live imaging and for fixed samples.
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Signup to use microscope. During covid rules of one person per room, must also block out Elyra 7 microscope. |
Instructions: [Word doc] [PDF] |
A few of the specifications:
The 60X planapochromat lens is the most versatile for most high resolution imaging. With the Zyla camera it oversamples (the equivalent of a 100X lens). Phase contrast makes it ideal for cell culture imaging. It should be used with a #1.5 coverglass The 100X lens is designed for TIRF, but TIRF was decomissioned in later 2019. The lens if best used for imaging where the sample is within a few um of a #1.5 coverglass.
Use this work-around to fix a bug in fluorescence timelapse imaging where the LED power resets to zero.
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Old website here for reference: index_prejuly2018.html |