Zeiss 800 Smilow 12

Inverted scope with AiryScan

Advance scheduling:


760 nm SP filter installed 7 October 2019


General Confocal Best practices:

Highly recommended reading: Tutorial: guidance for quantitative confocal microscopy


Zeiss claims that the microscope is prealigned ideally.
However, we have found that AiryScan should be aligned at each imaging session because the prealignment does not match all samples.
So you can see what alignment entails, instructions for the Zeiss 880 are here, but the 880 uses different software and using this microscope, the alignment tools are hidden in the Maintain tab to prevent users form altering the preset alignment.


If you need an explanation how to use the 63X lens to achieve full resolution, please ask Michael or Yan.
In brief: