This microscope has a monochrome camera and lenses with minimal focus shift due to color for high quality fluorescence imaging. It also has an automated stage for tiling large areas and a focus motor for Z series collection. It has DIC optics for transmitted light imaging. All lenses require #1.5 coverslip (170 to 175 um thick glass).
A few of the specifications:
People often ask, what about 100X? The 63X N.A. 1.40 lens has the same spatial resolution at the 100X lens when matched with the small pixel AxioCam on this microscope but with benefits such as a wider field of view.
Note about chromatic aberration. Different wavelengths focus at different focal planes. These lenses aim to correct this by being planapochromat. However, they are not perfect. For instance, we found a 2 um shift in Z from dapi to Cy5 with the 20X lens in a sample mounted in Prolong Diamond. To correct this, the Cy5 channel was set as the reference, precise focus is done in this channel, and a 2 um offset was set in the dapi channel. (Nomarski is imaged without an offset.)
Antivibration table needs to be floating properly for high resolution microscopy. Please turn off the gas after each use.
How to set the correct exposures for widefield fluorescence.
Typically images are too bright with too many saturated pixels. This undermines spatial resolution and the ability to quantify relative numbers of molecules. This video explains how to set good exposures.
It assumes excitation intensity fixed at 100% and imagers will only change exposure time. There is no discussion of camera gain, binning, or field size. This video exclusively addresses exposure time and its influence on dynamic range and LUT adjustments to change image appearance without altering the raw data.
Do you find that your sample bleaches while you are setting up imaging conditions? This video explains how to avoid this.