Zeiss AxioObserver microscope
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This microscope is for widefield fluorescence, simple brightfield, Nomarski/DIC, crossed polarization (see below), and phase contrast. It has automated focus for Z series and precision stage for tiling large areas or repeatably imaging multiple positions. It may be used for live imaging and for fixed samples.
A few of the specifications:
There is no filter set for Alexa Fluor 594 but the filter set for Cy3 works well.
People often ask, what about 100X? The 63X N.A. 1.40 lens has the same spatial resolution at the 100X lens when matched with the small pixel AxioCam on this microscope but with benefits such as a wider field of view. Also, a magnification lens my be placed in the lightpath for spatial oversampling.
Example Images. Click on images for larger versions.
All the micrographs in this figure were taken with the AxioObserver: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9720609/figure/F3/
Old website here for reference: index_prejune2018.html