Zeiss AxioImager.M1       

MSB 530

(last updated 20160622_0844)

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If you are shooting brightfield, make sure you Köhler align:

If imaging by brightfield, phase contrast, or DIC/Nomarski, always set condenser for Kohler illumination.
1. Focus on sample
2. set condenser for BF
3. Close Field Diaphagm
4. Move condenser up and down using knob on right until center octagon is in focus
5. Center by turning two knobs on condenser.
6. Open field diaphragm to edge of visible field, not all the way.

More explanation of Köhler illumination here.




About the instrument:

5X NA 0.15 EC Plan-Neofluar Spatial Scale
10X NA 0.30 EC Plan-Neofluar 420340-9900 Spatial Scale
20X NA 0.50 EC Plan-Neofluar 420350-9900 Spatial Scale
40X NA 0.75 EC Plan-Neofluar 420360-9900 Spatial Scale
63X NA 1.25 EC Plan-Neofluar 420480-9900 (oil) Spatial Scale

For best images use #1.5 coverslips. The lenses are designed specifically for #1.5 coverslips. Says so right on the side of them.

Camera: AxioCam MRm Rev3, 12 bits grayscale
This means that the intensity range is from 0 to 4095.

Green (GFP, Alexa488, etc.    ex450-490   FT510   BP515-565 
Red (Cy3, RFP, etc)     BP560/40   FT585   BP630/75
Blue (DAPI etc):     G365   FT395   BP445/50
near infra-red (Cy5, Alexa647, etc) [click here]

Software: AxioVision
Only installed multidimensional acquire modules are:
  mosaic (tiling)
  (Z stack and other software modules not installed.)

Computer: 32 bit Windows 7 with 16 GB RAM

Example image of f-actin (red), tubulin (green), and DNA (blue) using the 40X N.A. 0.75 lens. Nuclei are approximately 10 um diameter.

Click here for full field view linear scaled raw data.
Click here for full field view local contrast enhanced.








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comments, questions, suggestions for this web page: Michael.Cammer@med.nyu.edu or mcammer@gmail.com