Top row: single optical section
Bottom row: deconvolved in Metamorph



Before 3D, each channel copied, median filtered 25 X 25 pixels, multiplied by 0.85, subtracted from raw data, red channel only median 1 X 1



Top scalebar from metadata in image file.
Bottom scalebar based on published size of bacteria found in three images on Internet.


Comparison with 60X N.A. 1.4 widefield imaging on Nikon microscope: