For years Chroma has been giving out fluorescent Plexiglas slides which are great for testing and demonstrating all sorts of things on microscopes.

We noticed in the last batch that a distinctively green one wasn't included and a yellow one was. Question, would this really matter? Answer, probably not. See below.

In photo below, the three slides at the bottom are in the new set and the green on at the top is from a set a few years ago.
At the right, the new yellow and old green one pictured.

Spectra of the two slides as measured with excitation by 488 nm laser and measured on Zeiss 880 confocal in lambda mode.

spreadsheet here





Other fun things with the Plexiglas:





Blast from the past before I knew about these slides or before they were made:

PDF version click here