Nikon upright
All images 1 X 1 binning with Retiga camera.
Each square is 10 X 10 um. Thorlabs calibration slide.
In the 4X lens picture, the big box is 1 mm wide (divided into 100 10 um boxes).
4X air lens |
10X air lens |
20X air lens - thresholding followed by skeletonizing yields 0.42 um/pixel
|
40X air lens |