Nikon upright

All images 1 X 1 binning with Retiga camera.

Each square is 10 X 10 um. Thorlabs calibration slide.

In the 4X lens picture, the big box is 1 mm wide (divided into 100 10 um boxes).

4X air lens

10X air lens

20X air lens - thresholding followed by skeletonizing yields 0.42 um/pixel


40X air lens

 

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