Examples of nuclear translocation assay. Any confocal microscope can be used for this.
These examples are cell cultures that were fixed and stained.
This may also be done on live cells which express fluorescent proteins or otherwise have vital labeling.
Quantification may be counts of nuclei which are positive vs negative or intensities per nucleus and per band of cytoplasm around each nucleus.
Leica SP2, 63X N.A. 1.4 lens, 2008
In the example below, the figure is a small cropped area inside 5 X 5 full field stitched tiles areas to automatically collect over 100 cells for quantification.
Zeiss LSM800, 40X N.A. 1.3 lens, 2014
