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This microscope is primarily for widefield fluorescence and Nomarski/DIC. It may image color brightfield by taking three sequential pictures with RGB filters (not recommended). It has automated focus for Z series and motorized stage for tiling large areas or repeatably imaging multiple positions. It may be used for live imaging and for fixed samples. Software modules include an eary
A few of the specifications:
Lenses:
- 4x/0.2 Plan Apo WD 20 mm
- 10x/0.45 Plan Apo WD4.0 mm
- 20X/0.45 ELWD DIC Plan FluorWD 7.4 mm
- 40X/0.60 ELWD DIC Plan FluorWD 3.7 mm (in drawer; not on scope)
- 40X/1.30 DIC Plan Fluor WD 0.2 mm
- 60X/1.40 DIC Apo WD 0.14 mm
Hardware:
- Coolsnap HQ2 camera, 1392 X 1040 pixels at 14 bits
- Automated stage
- early generation PFS (autofocus)
- Lumencor LEDs for excitation at 395, 440, 470, 508, 555, 640 nm
- Quad block for blue/green/red/far-red
- filter wheel in front of camera
(filters marked as narrow pass)
- single narrow pass blocks for blue, green, red, far-red
- Laser for ablation/photoactivation
- filter cube for multipoint ablation/photoactivation with GFP
imaging
- Point ablation/photoactivation
- whole scope body incubation chamber
Software:
- Nikon Elements 4.30.01 (Build 1021) 64 bits HASP ID 1386AC0D Demonstration license No upgrade limit
- Deconvolution including Autoquant
- HCA/JOBS (High Content Acquisition)
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