Goal:
To look at five fluorescent probes on confocal.

According to the following schematic of the emission spectra, one of the probes overlaps three of the others and, therefore, cannot be separated.

However, the middle one that overlaps broadly, which is Pacific Orange, is excited at 405 nm. Therefore, it may be imaged simultaneous with the blue probe and cleanly segregated from the other probes which are excited at 488, 561, and 633 nm. (And a far red probe excited at 405 nm could be added too for a sixth.)

Here are tracks set up on the Zeiss 880 to effect this scheme. If the AF488 spills into the red channel or the red into the far-red, then the red channel (ex 561nm, em 569-624nm) could be put into an additional track.

(To save time by reducing one moving part between tracks, in "Track1" the Plate could be set to MSB-405.)

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