| |
Spatial Scales
Camera set at 1X1 binning with full field 1024 X 1024 pixels.
If you open the images, make sure your browser display is set 1:1 or download & save the image before loading it into ImageJ or other analysis software,
| Objective Lens |
Type & N.A. |
Pixel Size |
Field Size |
| 5X |
Zeiss Plan-NeoFluar N.A. 0.15 |
1.17 um |
1198 X 1198 um |
| 10X |
Olympus Plan N N.A. 0.25 Ph1 |
0.65 um |
666 X 666 um |
| 20X |
Olympus UPlanSApo N.A. 0.75 |
0.32 um |
328 X 328 um |
| 40X |
Zeiss NeoFluar N.A. 1.3 |
0.15 um |
154 X 154 um |
| 60X |
Olympus PlanApo N N.A. 1.42 |
0.11 um |
113 X 113 um |
| 100X |
Zeiss Plan-ApoChromat N.A. 1.4 |
0.06 um |
61 X 61 um |
| 100X 1.6X eyepiece |
Zeiss Plan-ApoChromat N.A. 1.4 |
0.038 um |
39 X 39 um |
(not to scale)
Settings for fluorescent blocks turret under objectives.
- (Unlabeled, 3 holes, pictured above) Standard all 4 channels
- CFP-YFP-mCherry Live
- GFP-mCherry Live
- empty
- empty
- empty
comments, questions, suggestions regarding these web pages: Michael.Cammer@med.nyu.edu or michael@coxcammer.com
comments, questions, suggestions regarding the microscope: Yan.Deng@med.nyu.edu
|
