Single excitation at 405 nm and emission at 10 nm intervals.

Of course the red will be less intense than the green because it is inefficiently excited at 405 nm compared to the green.

20171213 using new Nikon A1 spectral confocal. (Located here on the web because illustrates same point that the 880 is capable of.)



ImageJ macros used to make images above directly from raw nd2 file from Nikon Elements.