Zeiss 710 MP microscope

Question: what is response of slider for setting MaiTai laser intensity with AOM?

The numbers have been distributed on the slider to provide a linear response based on slider position. Spreadsheet here.

Question: What are baseline powers delivered at the sample?

Laser intensities measured by resting power meter probe on 10X objective.
In live scan mode with zoom 22.   April 13, 2011

• 633 nm 1.15 mW MBS 488/543/633
• 543 nm 0.16 mW MBS 488/543/633
• 514 nm 0.04 mW MBS 488/543/633
• 488 nm 0.37 mW MBS 488/543/633
• 458 nm 0.15 mW MBS 458
• 458 nm 0.17 mW MBS 458/514
• 514 nm 1.20 mW MBS 458/514
• 458 nm 0.12 mW MBS 458/543
• 543 nm 0.17 mW MBS 458/543
• 405 nm 2.54 mW MBS-405

• 633 nm 1.3 mW MBS 488/543/633
• 543 nm 0.20 mW MBS 488/543/633
• 488 nm 0.30 mW MBS 488/543/633
• 405 nm 2.56 mW MBS-405

Laser intensities measured after new Ar laser installed and realignment July 2, 2012

• 633 nm 1.4 mW MBS 488/543/633
• 543 nm 0.2 mW MBS 488/543/633
• 514 nm 0.0 mW MBS 488/543/633
• 488 nm 0.9 mW at max; 0.6 mW standby MBS 488/543/633
• 458 nm 0.3 mW at max; 0.2 mW standby MBS 458
• 405 nm 2.3 mW MBS-405

Laser intensities measured with Holger's power meter 20131220:

• 405 nm 1.45 mW -405 splitter alone
• 488 nm 0.169 mW 488/543/633 splitter alone; laser set to lowest standby power

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comments, questions, suggestions: Michael.Cammer@med.nyu.edu